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Cutting tool form compensation system and method. Barkman, W.E.; Babelay, E.F. Jr.; Klages, E.J. A compensation system for a computer-controlled machining apparatus having a controller and including a cutting tool and a workpiece holder which are movable relative to one another along a preprogrammed path during a machining operation utilizes a camera and a vision. Yucasin Treatment. Newly formed rhizomes from CXF and CSQ were prepared as described in the “Plant Material” section, and inoculated in petri dishes containing SIM supplemented with 200 μM yucasin (cat. 573760, Sigma–Aldrich, St. Louis, MO, United States) (Nishimura et al., 2014). Fifteen CSQ rhizomes were inoculated on one side of the. Powerful Servo System Configuration. Set up all the servo amplifiers in an entire machine from the intuitive interface of SigmaWin+ Ver. Follow the wizards to optimize setup ease, or take detailed control of individual operating parameters on any SERVOPACK in the system. Search results for 88.1 at Sigma-Aldrich. Compare Products: Select up to 4 products.Please select more than one item to compare.

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Yucasin Sigma Lambda

Yucasin Sigma

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Yucasin Sigma Theta

School of Science
Department of Biological Sciences
Laboratories
Molecular Neuroscience
Developmental Biology
Cellular Genetics
Molecular Genetics
Plant Development and Physiology
Cellular Biochemistry
Neurobiology
Evolutionary Genetics
Plant Environmental Responses
Environmental Microbiology
Animal Ecology
Plant Ecology
Systematic Zoology
Systematic Botany
Photosynthetic Microbial Consortia
'How many genes are essential for the simplest cellular organism to live?' 'What are the functions of the genes included in the minimum gene set?' We are trying to answer these questions using the bacteria, Escherichia coli, which is well understood and can be investigated in detail at the molecular level.
ProfJun-ichi Katoe-mail
Asc ProfShigeki Ehirae-mail
To identify the minimum set of genetic information which is essential for cell proliferation, several types of long chromosomal deletions were systematically constructed. Using these long chromosomal deletions, we identified all of the essential genetic information including small essential genes and essential chromosome regions encoding no proteins or no RNA. The deletion mutations we have constructed cover more than 90% of the whole genome, and during construction of these deletions, we identified novel essential genes. We also succeeded in constructing an E. coli strain that lacks about 30% of the parental chromosome; there are no organisms which have such significantly reduced genome. Our final goal is to construct 'minimum E. coli' which has the minimum gene set.
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Systematic Construction of E. coli deletion mutants
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Functional analysis of non-characterized essential genes
We are trying not only to identify all of the essential genes but also to clarify their cellular functions. Our first approach to understanding the functions of the non-characterized essential genes is genetic analysis. Isolation of their mutants (temperature-sensitive mutants) and analyses of their phenotypes at the non-permissive temperature sometimes provide clues, and further isolation of suppressor mutants may enable identification of functionally relevant genes and yield hints. On the basis of these genetic analyses, biochemical studies at the molecular level are necessary to understand in detail the activity of the gene products. We are especially interested in the mechanism of chromosome replication, partition and cell division. We have identified many important essential factors, topoisomerase IV, Hda protein and so on. Recently, we have also investigated the regulatory mechanism of gene expression and RNA degradation.
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Identification of essential DNA topoisomerase, topo IV
Regulatory network of cellular differentiation in cyanobacteria
Cyanobacteria are a large and morphologically diverse group of phototrophic prokaryotes with wide ecological tolerance that they occur in almost every habitat on earth. Some cyanobacteria can use not only CO2 as a carbon source by photosynthesis, but also N2 as a nitrogen source by nitrogen fixation. Filamentous cyanobacteria, such as Anabaena sp. strain PCC 7120, form differentiated cells called heterocysts, which are specialized cells for nitrogen fixation. Upon limitation of combined nitrogen in the medium, particular vegetative cells within linear multicellular filaments differentiate into heterocysts with a regular spacing of 10-15 cells. Heterocyst differentiation takes about 24 h to complete, when approximately 10% of chromosomal genes are upregulated with spatiotemporal regulation. We are analysing the molecular mechanisms of cellular differentiation and pattern formation using cyanobacteria.
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PEC (Profiling of E. coli chromosome)
  1. Shimmori, Y., Kanesaki, Y., Nozawa, M., Yoshikawa, H., Ehira, S. (2018) Transcriptional activation of glycogen catabolism and the oxidative pentose phosphate pathway by NrrA facilitates cell survival under nitrogen starvation in the cyanobacterium Synechococcus sp. strain PCC 7002. Plant Cell Physiol. in press. doi:10.1093/pcp/pcy059
  2. Ehira, S., Takeuchi, T, Higo, A. (2018) Spatial separation of photosynthesis and ethanol production by cell type-specific metabolic engineering of filamentous cyanobacteria. Appl. Microbiol. Biotechnol. 102:1523-1531.
  3. Higo, A., Isu, A., Fukaya, Y., Ehira, S., Hisabori, T. (2018) Application of CRISPR interference for metabolic engineering of the heterocyst-forming multicellular cyanobacterium Anabaena sp. PCC 7120. Plant Cell Physiol. 59:119-127.
  4. Iwadate, Y. and Kato, J. (2017) Involvement of the ytfK gene from the PhoB regulon in stationary-phase H2O2 stress tolerance in Escherichia coli. Microbiology. 163: 1912-1923.
  5. Iwadate, Y., Funabasama, N. and Kato, J. (2017) Involvement of formate dehydrogenases in stationary phase oxidative stress tolerance in Escherichia coli. FEMS Microbiology Letter. 364(20). doi: 10.1093/femsle/fnx193.
  6. Ehira, S., Shimmori, Y., Watanabe, S., Kato, H., Yoshikawa, H., Ohmori, M. (2017) The nitrogen-regulated response regulator NrrA is a conserved regulator of glycogen catabolism in ƒÀ-cyanobacteria. Microbiology 163:1711-1719.
  7. Fujisawa, T., Narikawa, R., Maeda, SI., Watanabe, S., Kanesaki, Y., Kobayashi, K., Nomata, J., Hanaoka, M., Watanabe, M., Ehira, S., Suzuki, E., Awai, K., Nakamura, Y. (2017) CyanoBase: A large-scale update on its 20th anniversary. Nucleic Acids Res. 45:D551-D554.
  8. Watanabe, K., Tominaga, K., Kitamura, M., and Kato, J. (2016) Systematic identification of synthetic lethal mutations with reduced-genome Escherichia coli: synthetic genetic interactions among yoaA, xthA and holC related to survival from MMS exposure. Genes Genet. Syst. 91: 183-188.
  9. Ohbayashi, R., Watanabe, S., Ehira, S., Kanesaki, Y., Chibazakura, T., Yoshikawa, H. (2016) Diversification of DnaA dependency for DNA replication in cyanobacterial evolution. ISME J. 10:1113-1121.
  10. Kurata, T., Nakanishi, S., Hashimoto, M., Taoka, M., Yamazaki, Y., Isobe, T., and Kato, J. (2015) Novel essential gene involved in 16S rRNA processing in Escherichia coli. J Mol. Biol. 427: 955-965.
  11. Ehira, S., Miyazaki, S. (2015) Regulation of genes involved in heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120 by a group 2 sigma factor SigC. Life 5:587-603.
  12. Nishimura, T., Hayashi, K., Suzuki, H., Gyohda, A., Takaoka, C., Sakaguchi, Y., Matsumoto, S., Kasahara, H., Sakai, T., Kato, J., Kamiya, Y., and Koshiba, T. (2014) Yucasin is a potent inhibitor of YUCCA, a key enzyme in auxin biosynthesis. The Plant Journal. 77: 352-366.
  13. Ehira, S., Kimura, S., Miyazaki, S., Ohmori, M. (2014) Sucrose synthesis in the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 is controlled by the two-component response regulator OrrA. Appl. Environ. Microbiol. 80:5672-5679.
  14. Halimatul, H.S.M., Ehira, S., Awai, K. (2014) Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120. Biochem. Biophys. Res. Commun. 450:178-183.
  15. Ehira, S. and Ohmori, M. (2014) NrrA directly regulates expression of the fraF gene and antisense RNAs for fraE in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Microbiology 160:844-850.
  16. Watanabe, M., Semchonok, D.A., Webber-Birungi, M.T., Ehira, S., Kondo, K., Narikawa, R., Ohmori, M., Boekema, E.J. and Ikeuchi, M. (2014) Attachment of phycobilisomes in an antenna-photosystem I supercomplex of cyanobacteria. Proc. Natl. Acad. Sci. USA 111:2512-2517.
  17. Hashimoto, C., Hashimoto, M., Honda, H., and Kato, J. (2013) Effects on IS1 transposition frequency of a mutation in the ygjD gene involved in an essential tRNA Modification in Escherichia coli. FEMS Microbiology Letter. 347: 140-148.
  18. Ehira, S. (2013) Transcriptional regulation of heterocyst differentiation in Anabaena sp. strain PCC 7120. Russ. J. Plant Physiol. 60:443-452.
  19. Kushige, H., H. Kugenuma, M. Matsuoka, S. Ehira, M. Ohmori, and H. Iwasaki. (2013) Genome-wide and heterocyst-specific circadian gene expression in the filamentous cyanobacterium, Anabaena sp. PCC 7120. J. Bacteriol. 195:1276-1284.
  20. Iwamoto, A., Osawa, A., Kawai, M., Honda, H., Yoshida, S., Furuya, N., and Kato, J. (2012) Mutations in the essential Escherichia coli gene, yqgF, and their effects on transcription. J. Mol. Microbiol. Biotechnol. 22: 17-23.
  21. Ehira, S., and M. Ohmori. (2012) The redox-sensing transcriptional regulator RexT controls expression of thioredoxin A2 in the cyanobacterium Anabaena sp. strain PCC 7120. J. Biol. Chem. 287:40433-40440.
  22. Ehira, S., and M. Ohmori. (2012) The pknH gene restrictively expressed in heterocysts is required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120. Microbiology 158:1437-1443.
  23. Tanaka, Y., S. Ehira, H. Teramoto, M. Inui, and H. Yukawa. (2012) Coordinated regulation of gnd encoding 6-phosphogluconate dehydrogenase by two transcriptional regulators GntR1 and RamA in Corynebacterium glutamicum. J. Bacteriol. 194: 6527-6536.
  24. Hashimoto, C., Sakaguchi, K., Taniguchi, Y., Honda, H., Oshima, T., Ogasawara, N., and Kato, J. (2011) Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes involved in a universal tRNA modification in Escherichia coli. J. Bacteriol. 193: 6075-6079.
  25. Iwadate, Y., Honda, H., Sato, H., Hashimoto, M. and Kato, J. (2011) Oxidative stress sensitivity of engineered Escherichia coli cells with a reduced genome. FEMS Microbiology Letter. 322: 25-33.
  26. Tachikawa, T., and Kato, J. (2011) Suppression of the temperature-sensitive mutation of the bamD gene required for the assembly of outer membrane proteins by multicopy of the yiaD gene in Escherichia coli. Biosci. Biotechnol. Biochem. 75: 162-164.
  27. Ehira, S., and M. Ohmori. (2011) NrrA, a nitrogen-regulated response regulator protein, controls glycogen catabolism in the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. J. Biol. Chem. 286:38109-38114.
  28. Ehira, S., H. Teramoto, M. Inui, and H. Yukawa. (2010) A novel redox-sensing transcriptional regulator CyeR controls expression of an old yellow enzyme family protein in Corynebacterium glutamicum. Microbiology 156:1335-1341.
  29. Yoshimura, H., Y. Kaneko, S. Ehira, S. Yoshihara, M. Ikeuchi, and M. Ohmori. (2010) CccS and CccP are involved in construction of cell surface components in the cyanobacterium Synechocystis sp. strain PCC 6803. Plant Cell Physiol. 51:1163-1172.
  30. Fujisawa, T., R. Narikawa, S. Okamoto, S. Ehira, H. Yoshimura, I. Suzuki, T. Masuda, M. Mochimaru, S. Takaichi, K. Awai, M. Sekine, H. Horikawa, I. Yashiro, S. Omata, H. Takarada, Y. Katano, H. Kosugi, S. Tanikawa, K. Ohmori, N. Sato, M. Ikeuchi, N. Fujita, and M. Ohmori. (2010) Genomic structure of an economically important cyanobacterium, Arthrospira (Spirulina) platensis NIES-39. DNA Res. 17:85-103.
  31. Toyoshima, M., N. Sasaki, M. Fujiwara, S. Ehira, M. Ohmori, and N. Sato. (2010) Early candidacy for differentiation into heterocysts in the filamentous cyanobacterium Anabaena sp. PCC 7120. Arch. Microbiol. 192:23-31.
  32. Ehira, S., H. Ogino, H. Teramoto, M. Inui, and H. Yukawa. (2009) Regulation of quinone oxidoreductase by a redox-sensing transcriptional regulator QorR in Corynebacterium glutamicum. J. Biol. Chem. 284:16736-16742.
  33. Ehira, S., H. Teramoto, M. Inui, and H. Yukawa. (2009) Regulation of Corynebacterium glutamicum heat shock response by the extracytoplasmic-function sigma factor SigH and transcriptional regulators HspR and HrcA. J. Bacteriol. 191:2964-2972.
  34. Asai, H., S. Iwamori, K. Kawai, S. Ehira, J. Ishihara, K. Aihara, S. Shoji, and H. Iwasaki. (2009) Cyanobacterial cell lineage analysis of the spatiotemporal hetR expression profile during heterocyst pattern formation in Anabaena sp. PCC 7120. PLoS ONE 4:e7371.
  35. Ohmori, K., S. Ehira, S. Kimura, and M. Ohmori. (2009) Changes in the amount of cellular trehalose, the activity of maltooligosyl trehalose hydrolase, and the expression of its gene in response to salt stress in the cyanobacterium Spirulina platensis. Microbes Environ. 24:52-56.
  36. Inoue, A., Murata, Y., Takahashi, H., Tsuji, N., Fujisaki, S., and Kato, J. (2008) Involvement of an essential gene, mviN, in murein synthesis in Escherichia coli. J. Bacteriol. 190: 7298-7301.
  37. Mizoguchi, H., Sawano, Y., Kato, J., and Mori, H. (2008) Superpositioning of deletions promotes growth of Escherichia coli with a reduced genome. DNA Research 15: 277-284.
  38. Kato, J. and Hashimoto, M. (2008) Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome. Methods in Molecular Biology (Microbial Gene Essentiality - Protocols and Bioinformatics) 416: 279-293. (Osterman, A. L. and Gerdes, S. Y. (ed.), Humana Press, Totowa, New Jersey)
  39. Ehira, S., T. Shirai, H. Teramoto, M. Inui, and H. Yukawa. (2008) Group 2 sigma factor SigB of Corynebacterium glutamicum positively regulates glucose metabolism under conditions of oxygen deprivation. Appl. Environ. Microbiol. 74:5146-5152.
  40. Kato, J. and Hashimoto, M. (2007) Construction of consecutive deletions of the Escherichia coli chromosome, Mol. Syst. Biol. 3: Article number 132
  41. Ikeuchi, Y., Shigi, N., Kato, J., Nishimura, A., and Suzuki, T. (2006) Mechanistic insights into sulfur-relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions. Mol Cell 21: 97-108.
  42. Ote, T., Hashimoto, M., Ikeuchi, Y., Su'etsugu, M., Suzuki, T., Katayama, T., and Kato, J. (2006) Involvement of the Escherichia coli folate-binding protein YgfZ in RNA modification and regulation of chromosomal replication initiation . Mol. Microbiol. 59: 265-275.
  43. Ehira, S., and M. Ohmori. (2006) NrrA directly regulates expression of hetR during heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 188:8520-8525.
  44. Ehira, S., and M. Ohmori. (2006) NrrA, a nitrogen-responsive response regulator facilitates heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Mol. Microbiol. 59:1692-1703.
  45. Kato, J. (2005) Regulatory network of the initiation of chromosomal replication in Escherichia coli. Crit. Rev. Biochem. Mol. Biol. (Critical Reviews in Biochemistry and Molecular Biology) 40: 331-342.
  46. Ikeuchi, Y., Soma, A., Ote, T., Kato, J., Sekine, Y., and Suzuki, T. (2005) Molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition. Mol. Cell 19: 235-246.
  47. Hashimoto, M., Ichimura, T., Mizoguchi, H., Tanaka, K., Fujimitsu, K., Keyamura, K., Ote, T., Yamakawa, T., Yamazaki, Y., Mori, H., Katayama, T. and Kato, J. (2005) Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome. Mol. Microbiol. 55: 137-149.
  48. Soma, A., Ikeuchi, Y., Kanemasa, S., Kobayashi, K., Ogasawara, N., Ote, T., Kato, J., Watanabe, K., Sekine, Y., and Suzuki, T. (2003) An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA. Mol. Cell 12:689-698.
  49. Hashimoto M, Kato J. (2003) Indispensabilityof the Escherichia coli carbonic anhydrases YadF and CynT in cell proliferation at a low CO2 partial pressure. Biosci Biotech Biochem. 67: 919-922.
  50. Ehira, S., M. Ohmori, and N. Sato. (2003) Genome-wide expression analysis of the responses to nitrogen deprivation in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. DNA Res. 10:97-113.
  51. Kato, J. and Katayama, T. (2001) Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli. EMBO Journal 20: 4253-4262.
  52. Kato, J., Fujisaki, S., Nakajima, K., Nishimura, Y., Sato, M., and Nakano, A. (1999) The E. coli homologue of yeast Rer2, a key enzyme of dolichol synthesis, is essential for carrier lipid formation in bacterial cell wall synthesis. J. Bacteriol. 181: 2733-2738.
  53. Tsukamoto, Y., Kato, J., and Ikeda, H. (1997) Silencing factors participate in DNA repair and recombination in Saccharomyces cerevisiae. Nature 388: 900-903.
  54. Shimizu, H., Yamaguchi, H., Ashizawa, Y., Kohno, Y., Asami, M., Kato, J., and Ikeda, H. (1997) Short-homology-independent illegitimate recombination in Escherichia coli: distinct mechanism from short-homology-dependent illegitimate recombination. J. Mol. Biol. 266: 297-305.
  55. Yokochi, T., Kato, J., and Ikeda, H. (1996) DNA nicking by Escherichia coli topoisomerase IV with a substitution mutation from tyrosine to histidine at the active site. Genes to Cells 1: 1069-1075.
  56. Kato, J., Suzuki, H., and Ikeda, H. (1992). Purification and characterization of DNA topoisomerase IV in Escherichia coli. J. Biol. Chem. 267: 25676-25684.
  57. Kato, J., Nishimura, Y., Imamura, R., Niki, H., Hiraga, S., and Suzuki, H. (1990). New topoisomerase essential for chromosome segregation in E. coli. Cell 63: 393-404.
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